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master's thesis
Određivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina
2022. urn:nbn:hr:109:388501
Miličević, Iva
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Food and Nutrition Research
Sub-department of Food Quality

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Miličević, I. (2022). Određivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:388501

Miličević, Iva. "Određivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. https://urn.nsk.hr/urn:nbn:hr:109:388501

Miličević, Iva. "Određivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. https://urn.nsk.hr/urn:nbn:hr:109:388501

Miličević, I. (2022). 'Određivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 16 April 2024, https://urn.nsk.hr/urn:nbn:hr:109:388501

Miličević I. Određivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2022 [cited 2024 April 16] Available at: https://urn.nsk.hr/urn:nbn:hr:109:388501

I. Miličević, "Određivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:109:388501

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TitleOdređivanje profila masnih kiselina, udjela ukupnih polifenola i antiradikalne aktivnosti morskih organizama Petrosia ficiformis i Bugula neritina
Title (english)Determination of Fatty Acid Profile, Total Polyphenol Content and Antiradical Activity of Marine Organisms Petrosia ficiformis and Bugula neritina
AuthorIva Miličević
MentorIvana Flanjak (mentor)
MentorStela Jokić (komentor)
MentorAna-Marija Cikoš (pomoć pri izradi, neposredni voditelj)
Committee memberKrunoslav Aladić (predsjednik povjerenstva)
Committee memberIvana Flanjak (član povjerenstva)
Committee memberStela Jokić (član povjerenstva)
Committee memberBlanka Bilić Rajs (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Food and Nutrition Research)
(Sub-department of Food Quality)
Osijek
Defense date and country2022-12-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Cilj ovog diplomskog rada bio je odrediti profil masnih kiselina, udio ukupnih polifenola i antiradikalnu aktivnost morskih organizama Jadranskog mora, spužve Petrosia ficiformis (izronjena kod poluotoka Rtina kod Paškog mosta) i mahovnjaka Bugula neritina (izronjen u uvali Luka, Dugi otok). Za ekstrakciju lipida korištena je metoda po Folch-u, a za identifikaciju masnih kiselina korištena je plinska kromatografija s plamenoionizacijskim detektorom. Ukupni polifenoli su ekstrahirani primjenom ultrazvuka, te je određen udio polifenola spektrofotometrijski pomoću Folin-Ciocalteuovog reagensa. DPPH metodom je određena antiradikalna aktivnost. Na temelju dobivenih rezultata utvrđeno je da su u P. ficiformis najzastupljeniji cis- i trans- izomeri oleinske kiseline (25,34 ± 0,26 %), a u B. neritina dominira palmitinska kiselina (48,38 ± 0,44 %). Također je uočeno da P. ficiformis (72,19 μg GAE/mL) ima veći udio ukupnih polifenola, nego B. neritina (59,01 μg GAE/mL). Određivanjem antiradikalne aktivnosti ultrazvučnom ekstrakcijom, na 30 °C su dobivene najveće vrijednosti za P.ficiformis (93,57 ± 0,70 % DPPH) i za B.neritina (93,65 ± 0,51 % DPPH). Povećanjem temperature smanjivala se antiradikalna aktivnost za oba uzorka.
Abstract (english)
The aim of this study was to determine the profile of fatty acids, total polyphenol content and antiradical activity in marine organisms from the Adriatic Sea Petrosia ficiformis and Bugula neritina. The sponge P. ficiformis was surfaced near the Rtina peninsula near the Paški bridge, and the bryozoa B. neritina was surfaced in the Luka cove, Dugi otok. The Folch method was used for the extraction of total lipids, and gas chromatography with flame-ionization detector was used for fatty acid identification. Total polyphenols were extracted using ultrasound, and polyphenol content was determined spectrophotometrically using the Folin- Ciocalteu reagent. The antiradical activity was determined by the DPPH method. Based on the obtained results, it was determined that cis- and trans- isomers of oleic acid (25.34 ± 0.26 %) are the most abundant in the sponge P. ficiformis, and palmitic acid (48.38 ± 0.44 %) dominates in bryozoan B. neritina. It was also observed that P. ficiformis (72.19 μg GAE/mL) has a higher content of total polyphenols than B. neritina (59.01 μg GAE/mL). By determining the antiradical activity by ultrasonic extraction, the highest values were obtained at 30 °C for P. ficiformis (93.57 ± 0.70 % DPPH) and for B. neritina (93.65 ± 0.51 % DPPH). Increasing the temperature decreased the antiradical activity for both samples.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:388501
ProjectNumber: KK.01.1.1.01.0002
Title: Bioprospecting Jadranskog mora
Leader: Rozelindra Čož Rakovac
Jurisdiction: eu
Funder: Europska unija
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) icon
Created on2023-01-10 16:36:41