prikaz prve stranice dokumenta Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus
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doctoral thesis
Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus
2014. urn:nbn:hr:109:619825
Šarkanj, Bojan
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology

Cite this document

Šarkanj, B. (2014). Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus (Doctoral thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:619825

Šarkanj, Bojan. "Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2014. https://urn.nsk.hr/urn:nbn:hr:109:619825

Šarkanj, Bojan. "Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2014. https://urn.nsk.hr/urn:nbn:hr:109:619825

Šarkanj, B. (2014). 'Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus', Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 18 April 2024, https://urn.nsk.hr/urn:nbn:hr:109:619825

Šarkanj B. Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus [Doctoral thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2014 [cited 2024 April 18] Available at: https://urn.nsk.hr/urn:nbn:hr:109:619825

B. Šarkanj, "Utjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus", Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:109:619825

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Metadata
TitleUtjecaj inhibitora glutation S – transferaze na produkciju aflatoksina plijesni Aspergillus flavus
Title (english)Effect of glutathione S – transferase inhibitors on aflatoxin production by Aspergillus flavus
AuthorBojan Šarkanj
MentorTomislav Klapec (mentor)
Committee memberIvan Kosalec (predsjednik povjerenstva)
MBZ: 218392
Committee memberTomislav Klapec (član povjerenstva)
Committee memberIvica Strelec (član povjerenstva)
Committee memberDaniela Čačić Kenjerić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Biochemistry and Toxicology)
Osijek
Defense date and country2014-06-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Food Chemistry
Universal decimal classification
(UDC)		615 - Pharmacology. Therapeutics. Toxicology
Abstract
Glutation S-transferaza (GST) je enzim s višestrukom funkcijom kojim se eukarioti mogu braniti od štetnog djelovanja metabolički aktiviranih aflatoksina, ali i reaktivnih vrsta kisika. Utvrđeno je da se kod plijesni povećava aktivnost GST-a kod povećane produkcije aflatoksina. Cilj ovog rada je bio ispitivanje utjecaja odabranih inhibitora ligandinske i katalitičke domene GST-a u stanici plijesni Aspergillus flavus na biosintezu aflatoksina. Rezultati su pokazali da inhibitori GST-a inhibiraju i produkciju aflatoksina u mikrobiološkoj podlozi u in vitro uvjetima kod svih ispitivanih koncentracija (10; 1; 0,1; 0,01 ppm), ali plijesni preživjele na podlozi s dodanim inhibitorima, imaju povišenu aktivnost GST-a, glutation reduktaze te katalaze. Ujedno, utvrđeni su dodatni sekundarni metaboliti koje ova plijesan proizvodi na ispitivanoj mikrobiološkoj podlozi LC-ESI-MS/MS analizom (QTrap). Uz osnovne intermedijere aflatoksina detektirane su ciklopijazonična, 3-nitropropionska te kojična kiselina Podešeni su odgovarajući parametri za primjenu ultrazvuka kao sigurnije i jeftinije metode razbijanja stanica plijesni. Također je praćena ekspresija regulatornih gena za biosintezu aflatoksina (aflR i aflS), pri čemu uz dodatak inhibitora GST-a dolazi do smanjenja ekspresije regulatornih gena te posljedične redukcije biosinteze aflatoksina.
Abstract (english)
Glutathione S-transferase (GST) is used in eukaryotic cell as defence enzyme from harmful effect of metabolically activated aflatoxin form. It is known that GST is positively correlated with aflatoxin biosynthesis. The aim of this thesis is to determine the effect of several GST inhibitors (both catalytic and ligand site) on aflatoxin production n in A. flavus. Results have shown that GST inhibitors inhibit also the aflatoxin production at all tested concentrations (10; 1; 0.1; 0.01 ppm) in vitro on microbiological medium, but survived cells have higher GST, glutathione reductase and catalase activity. Also the knowledge on A. flavus secondary metabolites produced on GMS medium has been expanded by LC-ESI-MS/MS (QTrap) analysis. In addition to basic aflatoxin biosynthesis pathway intermediates, cyclopiazonic acid, 3-nitropropionic acid, and kojic acid were detected. Ultrasonic disintegration method was also adjusted for mycelium as a safer and cheaper alternative of cell disruption. The expression of aflatoxin biosynthesis regulatory genes (aflR and aflS) was monitored and a lower expression followed addition of GST inhibitorsresulting in a lower aflatoxin biosynthesis rate.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:619825
Study programmeTitle: Food Engineering
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje prehrambena tehnologija (doktor/doktorica znanosti, područje biotehničkih znanosti, polje prehrambena tehnologija)
Type of resourceText
Extent257 str.; 30 cm
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2015-12-09 08:59:09