In addition to the animals and plants, microorganisms represent most numerous and most diverse group of living things on earth. To this world belongs: bacteria, fungi, algae, protozoa, viruses and other heterogeneous organisms different in their structure and life activities. According to their composition can be single-celled microorganisms (bacteria and some algae), multicellular (most species of fungi) or acellular - acellular material (viruses). The growth of microorganisms involves increasing the size of their cells, but also increase their number after reproduction, making the formation of colonies. There are many different factors related to the growth and reproduction of microorganisms, for example, preferred food, energy, atmospheric and temperature conditions. Nutrients or artificial surfaces are a mixture of different substances that allow propagation and maintenance of the vital functions of microorganisms under artificially created conditions. The culture medium should create conditions for microorganisms that are similar to the conditions in which they live when they are in their environment inside of a living organism or outside the organism. Condition of the culture medium on which they can develop all kinds of microorganisms, does not exist, because of the demands of certain microorganisms for different meals. Therefore, it is required to prepare a variety of substrates.