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DNA isolation from Aspergillus flavus : Optimal method selection
Croatian journal of food science and technology, 10 (2018), 2; 157-163. https://doi.org/10.17508/CJFST.2018.10.2.02
Šarkanj, Bojan; Bošnjak, Zinka; Perić, Magdalena; Kovač, Tihomir; Džijan, Snježana

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Šarkanj, B., Bošnjak, Z., Perić, M., Kovač, T. i Džijan, S. (2018). DNA isolation from Aspergillus flavus : Optimal method selection. Croatian journal of food science and technology, 10. (2), 157-163. doi: 10.17508/CJFST.2018.10.2.02

Šarkanj, Bojan, et al. "DNA isolation from Aspergillus flavus : Optimal method selection." Croatian journal of food science and technology, vol. 10, br. 2, 2018, str. 157-163. https://doi.org/10.17508/CJFST.2018.10.2.02

Šarkanj, Bojan, Zinka Bošnjak, Magdalena Perić, Tihomir Kovač i Snježana Džijan. "DNA isolation from Aspergillus flavus : Optimal method selection." Croatian journal of food science and technology 10, br. 2 (2018): 157-163. https://doi.org/10.17508/CJFST.2018.10.2.02

Šarkanj, B., et al. (2018) 'DNA isolation from Aspergillus flavus : Optimal method selection', Croatian journal of food science and technology, 10(2), str. 157-163. doi: 10.17508/CJFST.2018.10.2.02

Šarkanj B, Bošnjak Z, Perić M, Kovač T, Džijan S. DNA isolation from Aspergillus flavus : Optimal method selection. Croatian journal of food science and technology [Internet]. 30.11.2018. [pristupljeno 22.01.2025.];10(2):157-163. doi: 10.17508/CJFST.2018.10.2.02

B. Šarkanj, Z. Bošnjak, M. Perić, T. Kovač i S. Džijan, "DNA isolation from Aspergillus flavus : Optimal method selection", Croatian journal of food science and technology, vol. 10, br. 2, str. 157-163, Studeni 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:109:465854. [Citirano: 22.01.2025.]

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Podaci o radu
Naslov (engleski)DNA isolation from Aspergillus flavus : Optimal method selection
AutorBojan Šarkanj
AutorZinka Bošnjak
AutorMagdalena Perić
AutorTihomir Kovač
AutorSnježana Džijan
Autorova ustanovaSveučilište Josipa Jurja Strossmayera u Osijeku
Fakultet za dentalnu medicinu i zdravstvo Osijek
Autorova ustanovaSveučilište Josipa Jurja Strossmayera u Osijeku
Medicinski fakultet Osijek
Autorova ustanovaSveučilište Josipa Jurja Strossmayera u Osijeku
Prehrambeno-tehnološki fakultet Osijek
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Prehrambena tehnologija
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Kemija
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Biotehnologija
Sažetak (engleski)
The methods for fungal genomic DNA isolation for PCR amplification, including commercially available kits, must often be adapted in order to produce sufficient amounts of high-quality DNA from specific fungal species. The aim of this study was to select an optimal method for the isolation of DNA from Aspergillus flavus suitable for PCR reaction. Four different methods were compared according to their efficiency in isolating pure DNA, their price and time consumption. DNA quantification and purity estimation were performed using the NanoDropTM 1000 UV/VIS spectrophotometer and DNA integrity and PCR products were determined by gel-electrophoresis. DNA quantity ranged from 92.77 ± 11.52 to 5477.4 ± 22.03 ng/µL, with A260/280 from 1.14 ± 0.10 to 1.94 ± 0.16, and A260/230 0.37 ± 0.05 to 1.91 ± 0.17. There were also great differences in time consumption per sample, ranging from 1 hr 15 min to 7 hr 5 min. The determined costs per sample were ranging from 0.12 € to 2.29 € per sample. All tested methods were suitable for the isolation of A. flavus genomic DNA and subsequently for PCR reaction.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatian journal of food science and technology
Brojčani podacivol. 10, br. 2, str. 157-163
p-ISSN1847-3466
e-ISSN1848-9923
DOIhttps://doi.org/10.17508/CJFST.2018.10.2.02
URN:NBNurn:nbn:hr:109:465854
Datum objave publikacije2018-11-30
URL dokumentahttps://hrcak.srce.hr/file/308826
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Bez prerada 4.0 međunarodna (CC BY-ND 4.0)
Javna napomenaThis research was supported by the Josip Juraj Strossmayer University of Osijek (Young Scientist Grant). We would like to acknowledge the help and technical advice we received from Sanja Kuzmac, BSc, and Vedran Katavić, PhD, from the University of Zagreb, who provided us with the NanoDropTM.
Datum i vrijeme pohrane2025-01-17 15:51:48