This study investigated the ability of newly isolated white rot fungi Trametes versicolor (L.) and Trametes gibbosa (Pers.) Fr. to decolorize synthetic dyes (fuchsine, congo red and crystal violet). Prior to decolorization tests, qualitative test for determination of cellulolytic and lignolytic activity were performed and were positive for both fungi. The fungi were cultivated on agar plates containing dyes at concentrations of 10, 30, 50, 100 and 150 mg L\(^{-1}\) for 14 days. The radial growth and the zone of colour change on the agar plates were measured regularly every two days. The agar plates were then stored at room temperature and the change of colour was monitored visually for another 14 days. After 28 days, T. versicolor TV8 completely decolorize all agar plates containing congo red at all the applied concentrations. Apart from agar plates with the addition of 150 mg L\(^{-1}\) fuchsine and crystal violet, all other agar plates with the adition of fuchsine and crystal violet were successfully decolorized. T. gibbosacompletely decolorized all agar plates containing congo red except at the highest concentration of 150 mg L\(^{-1}\). Complete decolorization of agar plates containing fuchsine was only noted for concentrations of 10 and 30 mg L\(^{-1}\), while in case of crystal violet the complete decolorization only occurred on agar plates with added concentration of crystal violet of 10 mg L\(^{-1}\). Furthermore, T. versicolor was cultivated under solid-state conditions using congo red loaded brewers' spent grain as a carrier and substrate. T. versicolor grew readily under solid-state conditions, which was confirmed by the increase of protein content and substrate mass loss of 17% compared to abiotic control. Only partial decolorisation of congo red was observed, which was confirmed visually and by measuring the changes of color parameters.