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master's thesis
Istraživanje enzima prikladnih za razgradnju lignina
2020. urn:nbn:hr:109:474262
Ramljak, Sanja
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Bioprocess Engineering

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Ramljak, S. (2020). Istraživanje enzima prikladnih za razgradnju lignina (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:474262

Ramljak, Sanja. "Istraživanje enzima prikladnih za razgradnju lignina." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2020. https://urn.nsk.hr/urn:nbn:hr:109:474262

Ramljak, Sanja. "Istraživanje enzima prikladnih za razgradnju lignina." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2020. https://urn.nsk.hr/urn:nbn:hr:109:474262

Ramljak, S. (2020). 'Istraživanje enzima prikladnih za razgradnju lignina', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 26 June 2022, https://urn.nsk.hr/urn:nbn:hr:109:474262

Ramljak S. Istraživanje enzima prikladnih za razgradnju lignina [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2020 [cited 2022 June 26] Available at: https://urn.nsk.hr/urn:nbn:hr:109:474262

S. Ramljak, "Istraživanje enzima prikladnih za razgradnju lignina", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:109:474262

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Metadata
TitleIstraživanje enzima prikladnih za razgradnju lignina
Title (english)Elucidation of Enzymes Suitable for Lignin Degradation
AuthorSanja Ramljak
MentorNatalija Velić (mentor)
Committee memberIvica Strelec (predsjednik povjerenstva)
Committee memberNatalija Velić (član povjerenstva)
Committee memberHrvoje Pavlović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Bioprocess Engineering)
Osijek
Defense date and country2020-11-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kako bi se istražila enzimska razgradnja kraft lignina, određivana je aktivnost lakaze, najučinkovitijeg enzima u razgradnji lignina te aktivnosti tzv. “pomoćnih“ enzima glukoza dehidrogenaze (GDH), celobioza dehidrogenaze (CDH) i litičke polisaharid monooksigenaze (LPMO). Proveden je uzgoj gljiva bijelog truljenja Trametes versicolor i Phanerochaete chrysosporium kako bi se usporedila njihova učinkovitost razgradnje lignina s učinkovitosti gore navedenih enzima. Lakaza se pokazala učinkovitom u razgradnji lignina, dok pomoćni enzimi ne djeluju na lignin, ali pospješuju depolimerizaciju lignoceluloznih sirovina. Trametes versicolor se pokazala učinkovitom u razgradnji lignina što se dovodi u vezu s proizvodnjom lakaze.
Abstract (english)
Enzymatic degradation of kraft lignin was investigated using purified fungal enzymes known to degrade lignin structures. The activity of laccase, the most frequent enzyme associated with ligninolysis and the activity of auxiliary redox enzymes such as glucose dehydrogenase (GDH), cellobiose dehydrogenase (CDH) and lytic polysaccharide monooxygenase (LPMO) were tested. The effectiveness of these purified enzymes was compared with crude extracts obtained from fungal cultivations of the ligninolytic model fungi: Trametes versicolor and Phanerochaete chrysosporium. Laccase was proven to be the most efficient enzyme for lignin degradation. Auxiliary enzymes cooperate with other enzymes in order to enhance depolymerisation of lignocellulose. With observed high laccase activity, Trametes versicolor is found to be efficient in lignin degradation.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:109:474262
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Public noteizv. prof. dr. sc. Roland Ludwig - komentor
Created on2020-12-04 11:44:25