master's thesis
Protein content and enzyme activity of selected honey types

Katarina Sakoman (2012)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Food and Nutrition Research
Sub-department of Food Quality
Metadata
TitleUdio proteina i enzimska aktivnost izabranih vrsta meda
AuthorKatarina Sakoman
Mentor(s)Ljiljana Primorac (thesis advisor)
Abstract
Proteini, uključujući i enzime, u medu su prisutni u vrlo malim količinama i ne doprinose značajno nutritivnoj vrijednosti meda, ali su vrlo bitni parametri u procjeni kakvoće meda. Zadatak ovog rada bio je odrediti udio proteina, prolina i aktivnost enzima meda kestena, kadulje, bagrema i medljikovca kako bi se utvrdile specifičnosti pojedinih vrsta meda. Nakon provedene melisoplinološke analize, određeni su sljedeći fizikalno-kemijski parametri: udio vode, električna provodnost, udio HMF-a, sastav i udio ugljikohidrata, udio proteina i prolina te aktivnost invertaze, dijastaze, glukoza-oksidaze i kisele fosfataze. Ispitana je povezanost između pojedinih enzima, udjela proteina i prolina u medu. Tamnije vrste meda u pravilu su imale veći udio proteina i enzimsku aktivnost od svjetlijih vrsta s izuzetkom aktivnosti kisele fosfataze čije su vrijednosti izmjerene kod meda bagrema bile više nego kod meda kestena i kadulje. Osrednja do vrlo visoka statistički značajna korelacija utvrđena je između udjela proteina, prolina i analiziranih enzima, uz iznimku enzima kisele fosfataze. Vrlo visoka statistički značajna korelacija utvrđena je između dijastaze i invertaze dok su nešto niže statistički značajne korelacije utvrđene između glukoza-oksidaze i dijastaze te glukoza-oksidaze i invertaze.
Keywordshoney protein protein content enzyme enzyme activity
Parallel title (English)Protein content and enzyme activity of selected honey types
Committee MembersDaniela Čačić Kenjerić
Ljiljana Primorac
Ivica Strelec
Milena Mandić
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Food and Nutrition Research
Sub-department of Food Quality
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Food Safety and Quality
Study programme typeuniversity
Study levelgraduate
Study programmeFood Technology and Process Engineering
Academic title abbreviationdipl. ing.
Genremaster's thesis
Language Croatian
Defense date2012-10-11
Parallel abstract (English)
The content of proteins, including enzymes, in honey is relatively low and has a small nutritive significance. However, they are very important parameters for evaluation of the honey quality. The aim of this work was to determine protein and proline content, as well as enzyme activity of chestnut, sage, black locust and honeydew honey. After melissopalynological analysis was performed, following physico-chemical parameters were determined: water content, electrical conductivity, HMF content, carbohydrate content, protein and proline content, diastase, invertase, glucose-oxidase and acid phosphatase activity. The correlation between analysed enzyme activity, protein and proline content was studied. Generally, darker honey types had higher protein content and enzyme activity than lighter honey types with exception of acid phosphatase activity where black locust honey had higher activity than chestnut and sage honeys. Medium to very high statistically significant correlation was determined between protein content, proline content and analysed enzyme activities, with exception of acid phosphatase activity. Between diastase and invertase activity very high statistically significant correlation was determined, while slightly lower significant correlations were determined between glucose-oxidase, diastase and invertase activities.
Parallel keywords (Croatian)med proteini udio proteina enzimi enzimska aktivnost
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:631871
CommitterSanda Hasenay