master's thesis
Antifungal effect of thyme and tea tree essential oils

Maja Kovač (2011)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Food and Nutrition Research
Sub-department of Biology and Microbiology
Metadata
TitleAntifungalni učinak eteričnih ulja majčine dušice i čajevca
AuthorMaja Kovač
Mentor(s)Hrvoje Pavlović (thesis advisor)
Lidija Lenart (thesis advisor)
Abstract
Eterična ulja su aromatične tekućine iz biljnog materijala koje posjeduju manje ili više izražen antimikrobni učinak. Zbog sve veće potrebe za proizvodnjom namirnica bez sintetičkih aditiva, dodatak eteričnih ulja kao antimikrobnih i antifungalnih aditiva predstavlja veliki potencijal u proizvodnji mikrobiološki sigurnih namirnica. Zadatak rada bio je ispitati antifungalni učinak eteričnih ulja majčine dušice i čajevca na odabrane vrste plijesni Aspergillus carbonarius, A. flavus i A. parasiticus. Ispitana je minimalna inhibitorna (MIK) i minimalna fungicidna koncentracija (MFK) eteričnih ulja te učinak eteričnih ulja u inhibiciji linearnog rasta kolonije plijesni pri različitim vrijednostima aktiviteta vode (aw). Fungicidni učinak ulja čajevca se u svih ispitanih vrsta plijesni pokazao pri istoj koncentraciji od 20 000 ppm. Slični fungicidni učinak eteričnog ulja majčine dušice je primjećen na ispitane vrste roda Aspergillus uz iznimku vrste A. carbonarius koja se pokazala najosjetljivijom vrstom ovog roda plijesni budući je fungicidna koncentracija 10 000 ppm. Uspoređujući macrobroth i semisolid metodu, potonja se pokazala učinkovitijom budući su oba eterična ulja pokazala veća antifungalna svojstva pri nižim koncentracijama. Praćenjem linearnog rasta kolonija plijesni pri aw 0,95 i 0, 98, dodatak eteričnog ulja čajevca nije imao fungicidni učinak, no zamjećeno je produženje lag faze rasta, ovisno o koncentraciji. Dodatkom eteričnog ulja majčine dušice u podlogu za rast A. carbonarius, pri najvećoj koncentraciji ustanovljeno je fungicidno djelovanje. Djelovanje na A. flavus i A. parasiticus u slučaju oba ulja i obje vrijednosti aw je inhibitorno, budući usporava rast spomenutih plijesni.
Keywordsantifungal effect molds essential oils thyme tea tree
Parallel title (English)Antifungal effect of thyme and tea tree essential oils
Committee MembersSpomenka Kovač
Hrvoje Pavlović
Lidija Lenart
Nela Nedić Tiban
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Food and Nutrition Research
Sub-department of Biology and Microbiology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Food Microbiology
Study programme typeuniversity
Study levelgraduate
Study programmeFood Engineering
Academic title abbreviationmag. ing. techn. aliment.
Genremaster's thesis
Language Croatian
Defense date2011-05-31
Parallel abstract (English)
Essential oils are aromatic liquidsfrom plant material that possess more or less pronounced antimicrobial activity. Due to the increasing need for producing food without synthetic additives, the addition of essential oils as antimicrobial and antifugal additives represent a great potential in producing microbiologically safe foods. The task was to examine the antifungal activity of essential oil of thyme and tea tree oil on selected species of Aspergillus carbonarius, A. flavus and A. parasiticus. We examined the minimum inhibitory (MIC) and minimal fungicidal concentrations (MFK) of essential oils and the effect of essential oils in the inhibition of linear growth of mold colonies at different values of water activity (aw). Fungicidal effect of tea tree oil in all tested species of mold showed the same concentration of 20 000 ppm. Similar fungicidal effect of essential oil of thyme is observed on tested species of the genus Aspergillus, with the exception of A. carbonarius, which proved to be the most sensitive species of this genus of mold due to the fungicidal concentration of 10 000 ppm. Comparing macrobroth and semisolid method, the latter proved to be more proper since both essential oil showed greater antifungal properties at lower concentrations. During monitoring of linear growth of mold colonies in aw0.95 and 0. 98, supplement of essential tea tree oil had no fungicidal effect but there was extension in lag phase of growth, depending on the concentration. The addition of the highest concentration of thyme essential oil in A. carbonarius growth medium had fungicidal effect. Both essential oils had inhibitory effect while testing linear growth of A. flavus and A. parasiticus at aw values 0.95 and 0.98.
Parallel keywords (Croatian)antifugalni učinak plijesni eterična ulja majčina dušica čajevac
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:204706
CommitterSanda Hasenay