Groundwater is the main source of drinking water in eastern Croatia and they are usually enriched with humic substances and arsenic. Humic substances are the end product of decaying organic matter, and during chemical water disinfection with substances such as chlorine, harmful disinfection byproducts can be formed. Arsenic is, in the other hand, well known as a poison, and recent epidemiological studies confirmed its carcinogenic effect in human body. That why those compounds are need to be removed from drinking water during drinking water treatment. Adsorption is one of most used techniques for arsenic and humic substances removal. Some studies reports also good results in arsenic and humic substances removal using household water filtration system. The removal of arsenic and humic substance from drinking water of Osijek town were investigated using granular activated carbon (NORIT PK 1-3), anthracite (Hydraffin 30n), granular iron dioxide (GEH®), and household filtration system made of anthracite and granular iron dioxide. The impact of following parameters on removal efficiency was observed: pH value, electrical conductivity, arsenic concentration, chemical oxygen demand (COD) and specific UV absorbance at 254nm. Obtained results shown effective removal of humic substances using granulated activated carbon (>80%) and granular iron dioxide (60%). The lowest humic substances removal, up to 30%, was achieved by anthracite. Using household filtration system made of anthracite and granular iron dioxide, arsenic concentration was reduced for 20 to 45% from initial concentration detected in Osijek’s drinking water during this study.