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master's thesis
Proizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu
2023. urn:nbn:hr:109:617580
Srdoč, Antun
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering

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Srdoč, A. (2023). Proizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:617580

Srdoč, Antun. "Proizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. https://urn.nsk.hr/urn:nbn:hr:109:617580

Srdoč, Antun. "Proizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. https://urn.nsk.hr/urn:nbn:hr:109:617580

Srdoč, A. (2023). 'Proizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 28 September 2024, https://urn.nsk.hr/urn:nbn:hr:109:617580

Srdoč A. Proizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2023 [cited 2024 September 28] Available at: https://urn.nsk.hr/urn:nbn:hr:109:617580

A. Srdoč, "Proizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:109:617580

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Metadata
TitleProizvodnja hidrolitičkih enzima tijekom uzgoja Trametes versicolor na pivskom tropu
Title (english)Production of Hydrolytic Enzymes During Trametes versicolor Cultivation on Brewer's Spent Grain
AuthorAntun Srdoč
MentorMarina Tišma (mentor)
MentorAnđela Zeko-Pivač (pomoć pri izradi, neposredni voditelj)
Committee memberMirela Planinić (predsjednik povjerenstva)
Committee memberMarina Tišma (član povjerenstva)
Committee memberAna Bucić-Kojić (član povjerenstva)
Committee memberKristina Mastanjević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Thermodynamics and Reaction Engineering)
Osijek
Defense date and country2023-06-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Pivski trop je široko rasprostranjen nusproizvod pivarske industrije. U ovom radu proučavan je proces proizvodnje hidrolitičkih enzima (ksilanaza, invertaza, β‐glukozidaza i celulaza) u uvjetima fermentacije na čvrstim nosačima, koristeći pivski trop kao supstrat za uzgoj Trametes versicolor. Fermentacija na čvrstim nosačima provedena je pri sljedećim procesnim uvjetima: T = 27 °C, t = 15 dana, m (supstrata) = 30 g, udio vlage = 69,19 %, visina sloja: 4,5 cm. Gubitak na masi materijala nakon 15 dana fermentacije iznosio je 29 %, a pH supstrata kretao se od 5,40 do 6,47. Maksimalne volumne aktivnosti enzima ksilanaze i invertaze postignute su nakon 15. dana. Volumna aktivnost ksilanaze bila je V.A. = 511,19 U/mL, a invertaze V.A. = 186.25 U/mL. Maksimalna volumna aktivnost β‐glukozidaze postignuta je nakon 12. dana (V.A. = 4,34 U/mL), a celulaze nakon 10. dana (V.A. = 0,074 U/mL). Na temelju dobivenih rezultata dokazano je da je T. versicolor dobar mikroorganizam za proizvodnju istraživanih hidrolitičkih enzima tijekom uzgoja na pivskom tropu.
Abstract (english)
Brewer’s spent grain is a widespread by‐product of the brewing industry. In this work, the production process of hydrolytic enzymes (xylanase, invertase, β‐glucosidase, and cellulase) was studied under solid‐state fermentation (SSF) conditions, using brewer’s spent grain as substrate for the cultivation of Trametes versicolor. Solid‐state fermentation was performed under the following process conditions: T = 27 °C, t = 15 days, m (substrate) = 30 g, moisture content = 69.19%, layer height: 4.5 cm. The mass loss of the material after 15 days of fermentation was 29%, and the pH of the substrate ranged from 5.40 to 6.47. The highest volume activities of xylanase and invertase enzymes were reached after the 15th day, for xylanase V.A. = 511.19 U/mL and for invertase V.A. = 186.25 U/mL. The highest volume activity of β‐glucosidase was reached after day 12, V.A. = 4.34 U/mL, and of cellulase after day 10, V.A. = 0.074 U/mL. Based on the obtained results, it was proved that T. versicolor is a suitable microorganism for the production of investigated enzymes during cultivation on brewer’s spent grain.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:617580
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) icon
Created on2023-07-05 09:40:17