undergraduate thesis
SDS-PAGE of Barley Albumins and Globulins

Dragana Kajić (2013)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
Metadata
TitleSDS-PAGE albumina i globulina sorti ječma
AuthorDragana Kajić
Mentor(s)Ivica Strelec (thesis advisor)
Abstract
Cilj je ovog rada bio ustanoviti mogu li se sorte ječma međusobno razlikovati na osnovi rasporeda proteinskih vrpci albumina, globulina ili albuminsko/globulinske frakcije po provedenoj poliakrilamid gel elektroforezi u prisutnosti natrijeva dodecil-sulfata. Ekstrakcija proteina (albumina, globulina ili albuminsko/globulinske frakcije) provedena je na tri načina. Albumini i globulini su stupnjevito ekstrahirani pomoću Milli Q vode (albumini), i potom pomoću 0,5 M NaCl (globulini), a albuminsko/globulinska frakcija proteina pomoću dva otapala, 50 mM Tris-HCl pufera pH 8,0 ili 0,5 M otopine NaCl. Rezultati su pokazali da sorte ječma nije moguće nedvojbeno razlikovati na osnovi razlika u koncentraciji ekstrahiranih albumina, globulina ili albuminsko/globulinske frakcije proteina. Primjena SDS-PAGE albumina, globulina i albuminsko/globulinske frakcije proteina sorti ječma pokazala je ograničenu mogućnost razlikovanja sorti ječma. Najviše razlika između sorti uočeno je u elektroforetskom rasporedu albuminskih vrpci po provedenoj SDS-PAGE, gdje je nedvojbeno identificirano 3 od 6 ispitivanih sorti.
Keywordsbarley proteins electrophoresis
Parallel title (English)SDS-PAGE of Barley Albumins and Globulins
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelundergraduate
Study programmeFood Technology
Academic title abbreviationuniv. bacc. ing. techn. aliment.
Genreundergraduate thesis
Language Croatian
Defense date2013-09-23
Parallel abstract (English)
Discrimination of barley varieties by SDS-PAGE electrophoretic protein patterns of barley albumins, globulins and albumin/globulin protein fractions was investigated. Albumins and globulins were sequentially extracted from barley flour as follows: albumins by Milli Q water followed by extraction by 0.5 M NaCl for globulins, while albumin/globulin protein fractions were extracted directly by 50 mM Tris-HCl buffer pH 8.0 or 0.5 M NaCl. Obtained results showed limited capability for variety discrimination by protein content in albumin, globulin and/or albumin/globulin protein fractions, as well as by SDS-PAGE. The most promising was SDS-PAGE separation of albumins where 3 varieties of 6 examined were identified by their differences in protein pattern.
Parallel keywords (Croatian)proteini ječma elektroforeza
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:378790
CommitterIvana Šuvak