Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus

Brkić, Katarina

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master's thesis

Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus

2024. urn:nbn:hr:109:613058

Brkić, Katarina

Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering

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APA 6th Edition

Brkić, K. (2024). Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:613058

MLA 8th Edition

Brkić, Katarina. "Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2024. https://urn.nsk.hr/urn:nbn:hr:109:613058

Chicago 17th Edition

Harvard

Brkić, K. (2024). 'Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 03 February 2025, https://urn.nsk.hr/urn:nbn:hr:109:613058

Vancouver

Brkić K. Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2024 [cited 2025 February 03] Available at: https://urn.nsk.hr/urn:nbn:hr:109:613058

IEEE

K. Brkić, "Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:109:613058

Cite this item: https://urn.nsk.hr/urn:nbn:hr:109:613058

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Title	Proizvodnja, pročišćavanje i karakterizacija enzima endo-1,4-ß-ksilanaza iz Thermomyces lanuginosus
Title (english)	Production, Purification and Characterization of Enzyme endo-1,4-ß-xylanase from Thermomyces lanuginosus
Author	Katarina Brkić
Mentor	Marina Tišma (mentor)
Mentor	Anđela Matić (pomoć pri izradi, neposredni voditelj)
Committee member	Kristina Mastanjević (predsjednik povjerenstva)
Committee member	Marina Tišma (član povjerenstva)
Committee member	Mirela Planinić (član povjerenstva)
Committee member	Ana Bucić-Kojić (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek FACULTY OF FOOD TECHNOLOGY (Department of Process Engineering) (Sub-department of Thermodynamics and Reaction Engineering) Osijek
Defense date and country	2024-10-30, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Food Technology Engineering
Abstract	Enzim endo-1,4-β-ksilanaza se najčešće koristi u proizvodnji biogoriva, tvornicama pulpe i papira te u prehrambenoj industriji, zbog sposobnosti katalize hidrolize ksilana, sastavnog dijela hemiceluloze. Cilj ovog rada bio je proizvesti enzim endo-1,4-β-ksilanazu tijekom uzgoja Thermomyces lanuginosus metodom fermentacije na čvrstim nosačima, koristeći pivski trop kao supstrat. Tijekom 10 dana fermentacije svakodnevno su mjerene volumne i specifične aktivnosti enzima. Najveće aktivnosti postignute su osmi dan fermentacije (V.A = 3159,06 U/mL, S.A.= 6476,24 U/mg). Potom su istraživane različite metode pročišćavanja enzima: jednostupanjsko i dvostupanjsko taloženje u hladnom acetonu, taloženje amonijevim sulfatom s i bez dodatka t-butanola i dijaliza. Metodom elektroforeze određena je molekulska masa enzima koja je iznosila 12,5 kDa. Na kraju, provedena je analiza utjecaja pH i temperature na aktivnost pročišćenog enzima. Najveća aktivnosti enzima (V.A.= 20859,44 U/mL, S.A. = 7193,63 U/mg) izmjerena je pri pH 6 i temperaturi od 85 °C.
Abstract (english)	Enzyme endo-1,4-β-xylanase is mostly used in biofuel production, pulp and paper factories, as well as food industry because of its property to catalize hydrolytic reaction of xylan degradation, a compound found in hemicellulose. The purpose of this thesis is to produce the enzyme endo-1,4-β-xylanase by solid state fermentation of Thermomyces lanuginosus while using brewers' spent grain as a substrate. Firstly, volume and specific activities of the enzyme were measured daily during the 10 days of fermentation. The highest activity was achieved on the eight day (V.A.= 3159,06 U/mL, S.A. = 6476,24 U/mg). Secondly, purification of the enzyme was carried out by different methods: precipitation by cold acetone in one and two steps, precipitation by amonnium sulfate with and without addition of t-butanol and dialysis. Thirdly, the enzyme's molecule mass was determined by electrophorensis and was proven to be 12,5 kDa. In the end, the influence of pH and temperature on the purified enzyme's activity was measured. The highest enzyme activity (V.A.= 20859,44 U/mL, S.A. = 7193,63 U/mg) was gained at pH 6 and temperature of 85°C.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:109:613058
Project	Number: IPS-2023-02-6614 Title: Valorizacija proizvodnih ostataka iz tvornica ulja bazirana na konceptu biorafinerije Title: Valorisation of residues from the vegetable oil industry based on a biorefinery approach Acronym: VALREO Jurisdiction: Croatia Funder: Hrvatska zaklada za znanost Funding stream: Slovenian-Croatian Bilateral Projects
Study programme	Title: Process Engineering Study programme type: university Study level: graduate Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka procesnog inženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Created on	2024-11-04 17:19:11