undergraduate thesis
PCR in food diagnostic

Ivan Domazet (2009)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
Metadata
TitleLančana reakcija polimerazom (PCR) u dijagnostici hrane
AuthorIvan Domazet
Mentor(s)Vladimir Mrša (thesis advisor)
Abstract
PCR (eng. Polymerase Chain Reaction) je lančana reakcija polimerazom, metoda kojom se kratki dio DNA umnožava u veliki broj identičnih kopija bez korištenja živog organizma. Pronašao ju je Kary Mullis 1984. godine. Princip je vrlo jednostavan. Od jedne molekule DNA dobiju se dvije, nove, identične molekule te se taj postupak može ponavljati bezbroj puta. Znamo da svaki lanac DNA sadrži niz nukleotida koji je komplementaran suprotnom lancu u uzvojnici DNA. Pri replikaciji molekule DNA svaki lanac uzvojnice služi kao kalup za sintezu novog komplementarnog lanca. Sve se to zbiva uz pomoć enzima DNA-polimeraze. Kako bismo razumijeli PCR metodu potrebno je poznavati građu nukleinskih kiselina, njihov kemizam i samu ulogu u stanici. Postoje dva osnovna tipa nukleinskih kiselina: DNA i RNA. To su linearni polimeri koji se sastoje od različitih nukleotida poredanih određenim slijedom. PCR je našla primjenu u molekularnioj biologiji, mikrobilologiji, medicini, forenzici, te dijagnostici hrane kao što je određivanje vrste životinjskog mesa u hrani, mikroorganizama, mikotoksinogenih gljiva i dr.
KeywordsPCR DNA nucleic acids DNA-polymerase
Parallel title (English)PCR in food diagnostic
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelundergraduate
Study programmeFood Technology
Academic title abbreviationuniv. bacc. ing. techn. aliment.
Genreundergraduate thesis
Language Croatian
Defense date2009-09-30
Parallel abstract (English)
PCR (polimerase chain reaction) is a method which we use to amplify a piece of DNA by in vitro, developed in 1984 by Kary Mullis. The PCR method is very simple. We amplify a single molecule DNA across several orders of magnitude, generating millions or more copies of the DNA piece. It is known that each strand of DNA have sequence of nucleotides which is complementary to the opposite strain of DNA. During replication by the DNA polymerase each strand odf DNA molecule is used as template for synthesys of new strand. To understand PCR method, we should know the structure and function of nucleic acids. The most common nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). A nucleic acid is a macromolecule composed of chains of monomeric nucleotides. PCR is used in molecular biology and microbiology, medicine, forensics and food diagnostic such as detection of animal species in meat products, microorganisms, indentification of mycotoxigenic fungi etc.
Parallel keywords (Croatian)PCR DNA nukleinske kiseline DNA-polimeraza
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:886841
CommitterIvana Šuvak