master's thesis
Electrophoretic analysis of honey proteins

Lidija Brodar (2011)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
Metadata
TitleElektroforetske analize proteina meda
AuthorLidija Brodar
Mentor(s)Ivica Strelec (thesis advisor)
Abstract
Elektroforetskim analizama (Nativna PAGE, izoelektricno fokusiranje, SDS-PAGE) ispitana je mogucnost razlikovanja botanicki razlicitih vrsta meda na temelju njihovog proteinskog, te glikoproteinskog sastava. Detekcijom proteinskih vrpci nakon primjenjenih elektroforetskih metoda, te glikoproteina prenešenih na PVDF membranu nakon SDS-PAGE uoceno je da sve vrste meda pokazuju vrlo slicne proteinske i glikoproteinske elektroforetske profile koji se razlikuju samo po intenzitetu proteinskih vrpci. Prema intenzitetu proteinskih i glikoproteinskih vrpci izdvajaju se med bagrema koji pokazuje najmanji intenzitet, te med kestena, livadni med i bjelogoricna medljika (hrast) sa najjacim intenzitetima. Uklanjanje peludnih zrnaca nije utjecalo na elektroforetski proteinski profil nakon provedene nativne i SDS-PAGE, te izoelektricnog fokusiranja.
Keywordshoney proteins glycoproteins Native-PAGE SDS-PAGE isoelectric focusing
Parallel title (English)Electrophoretic analysis of honey proteins
Committee MembersLjiljana Primorac
Ivica Strelec
Daniela Čačić Kenjerić
Tomislav Klapec
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeFood Technology and Process Engineering
Academic title abbreviationdipl. ing.
Genremaster's thesis
Language Croatian
Defense date2011-09-15
Parallel abstract (English)
Possibility of discrimination of botanically different unifloral honeys by their protein and glycoprotein pattern obtained after Native PAGE, isoelectric focusing and SDS-PAGE was examined. All examined unifloral honeys shown similar protein and glycoprotein patterns which differentiated only in intensities of bands. According to differences in intensities of protein or glycoprotein bands the most notable were acacia honeys with the lowest protein or glycoprotein band intensities, and chestnut, meadow and honeydew (oak) honeys with the highest band intensities. Removal of pollen did not affected protein pattern of examined honeys obtained after SDS-PAGE and isoelectric focusing.
Parallel keywords (Croatian)med proteini glikoproteini SDS-PAGE nativna PAGE izoelektricno fokusiranje
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:208544
CommitterSanda Hasenay