master's thesis
Modulation of conditions of ultrasound homogenization for the disintegration of Aspergillus flavus mycelium

Ivan Meteš (2010)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
Metadata
TitleModulacija uvjeta ultrazvučne homogenizacije u razaranju micelija Aspergillus flavusa
AuthorIvan Meteš
Mentor(s)Tomislav Klapec (thesis advisor)
Abstract
Aspergillus flavus je široko rasprostranjena plijesan i najznačajniji producent aflatoksina. Produkcija aflatoksina povezuje se sa povišenom aktivnosti oksidoreduktivnih enzima plijesni. Cilj istraživanja bio je odrediti učinkovitost ultrazvučne homogenizacije te optimalne uvjete razbijanja micelija Aspergillus flavusa kako bi se u potpunosti oslobodio unutarstanični sadržaj, a da pri tome ne dođe do denaturacije proteina i gubitka aktivnosti katalaze. Na temelju dobivenih rezultata može se zaključiti da de se uz uz primjenu punog ciklusa i najviše amplitude (25 kHz) uređaja tijekom četiri minute, zadovoljavajude razoriti micelij plijesni sudedi po količini ekstrahiranih proteina. Najviša aktivnost katalaze utvrđena je nakon tri minute razaranja kod navedene frekvencije i punog ciklusa, te je ustanovljeno da dodatno razaranje taloga povedava ekstrakciju enzima. Oscilacije aktivnosti katalaze nakon tri minute upuduju na mogudnost gubitka enzimske aktivnosti samim tretmanom.
Keywordsmold Aspergillus flavus ultrasound homogenization proteins catalase
Parallel title (English)Modulation of conditions of ultrasound homogenization for the disintegration of Aspergillus flavus mycelium
Committee MembersIvica Strelec
Tomislav Klapec
Vinko Krstanović
Vedran Slačanac
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeFood Technology and Process Engineering
Academic title abbreviationdipl. ing.
Genremaster's thesis
Language Croatian
Defense date2010-12-17
Parallel abstract (English)
Aspergillus flavus mold is widespread and the most important producer of aflatoxin. Aflatoxin production is associated with increased activity of oxidoreductive enzymes in the mold. The aim of this study was to determine the effectiveness of ultrasonic homogenization and optimal conditions of disintegrating Aspergillus flavus mycelium in order to fully liberate intracellular content avoiding protein denaturation and loss of catalase activity. Based on the results, the implementation of the instruments’s full cycle and maximum amplitude (25 kHz) for four minutes led to satisfactory mold disintegration judging by the quantity of extracted proteins. The greatest catalase activity was determined after three minutes at the mentioned frequency and fully cycle and it was also established that additional sediment homogenization increases extraction of the enzyme. The vacillating catalase activity in samples treated longer than three minutes might indicate loss of enzyme activity caused by the treatment itself.
Parallel keywords (Croatian)plijesan Aspergillus flavus ultrazvučna homogenizacija proteini katalaza
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:508216
CommitterSanda Hasenay