master's thesis
Correlation between glutation S-transferase activity and aflatoxin synthesis in Aspergillus flavus

Andreja Čondor (2010)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
Metadata
TitleKorelacija aktivnosti glutation-S-transferaze plijesni Aspergillus flavus s opsegom sinteze aflatoksina
AuthorAndreja Čondor
Mentor(s)Tomislav Klapec (thesis advisor)
Abstract
U istraživanju je ispitivana aktivnost enzima glutation S-transferaze (GST) u odnosu na sintezu aflatoksina plijesni Aspergillus flavus, soj NRRL 3251 na različitim hranjivim podlogama. Korištene su GMS i PMS tekuće podloge koje sadrže različit izvor ugljika. Utvrđena je znatno veća količina aflatoksina na glukozom bogatoj GMS podlozi. Na PMS-u je izmjereno maksimalno 23,09 ppb ukupnih aflatoksina, dok na GMS-u maksimalan udio istih iznosi 362,14 ppb. Za analizu aflatoksina korištena je standardna metoda – imunoafinitetno pročišćavanje u koloni s HPLC analizom. Udio proteina veći je na PMS podlozi zbog prisutnosti peptona. Prema dobivenim rezultatima istraživanja, nije moguće utvrditi na kojoj je hranjivoj podlozi aktivnost GST-a veća. Također, nije utvrđena statistički značajna korelacija između sinteze aflatoksina i aktivnosti GST-a.
KeywordsAspergillus flavus aflatoxins glutathione S-transferase peptone glucose
Parallel title (English)Correlation between glutation S-transferase activity and aflatoxin synthesis in Aspergillus flavus
Committee MembersIvica Strelec
Tomislav Klapec
Hrvoje Pavlović
Vinko Krstanović
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeFood Technology and Process Engineering
Academic title abbreviationdipl. ing.
Genremaster's thesis
Language Croatian
Defense date2010-07-09
Parallel abstract (English)
Correlation between glutathione S-transferase activity (GST) and aflatoxin production by the mould Aspergillus flavus, strain NRRL 3251, grown on different culture media, was examined. GMS and PMS liquid culture media, containing diverse carbon sources, were used. A greater amount of total aflatoxins was determined in glucose rich GMS liquid Maximum total aflatoxin concentration in PMS medium was 23.09 ppb, while 362.14 ppb was measured in GMS. Standard methods of aflatoxin clean-up and detection were applied – immunoaffinity column chromatography combined with HPLC analysis. The quantity of protein was higher in PMS medium due to peptone content. According to the obtained results, it was not possible to estimate which culture media supported higher GST activity. Additionally, no statistically significant relationship between GST activity and aflatoxin production was determined.
Parallel keywords (Croatian)plijesni Aspergillus flavus aflatoksini glutation S- transferaza pepton glukoza
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:331998
CommitterSanda Hasenay