master's thesis
Syntesis of biodiesel by immobilized lipase from Thermomyces

Martina Lucić (2015)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering
Metadata
TitleSinteza biodizela pomoću imobilizirane lipaze iz Thermomyces lanuginosus
AuthorMartina Lucić
Mentor(s)Sandra Budžaki (thesis advisor)
Abstract
Cilj rada bio je imobilizirati lipazu iz Thermomyces lanuginosus na nosaču Eupergit CM i provesti sintezu biodizela pomoću imobilizirane lipaze u kotlastom reaktoru iz jestivog svježeg i otpadnog ulja. Proces imobilizacije koji je proveden na 24, 48, 72 i 96 h u dva pufera različite molarnosti (0,01 M i 0,1 M) najbolje je rezultate pokazao nakon imobilizacije na 72 h u 0,1 M fosfatnom puferu. Rezultati nakon provedene sinteze biodizela iz jestivog sviježeg i otpadnog ulja s imobiliziranom lipazom nisu bili zadovoljavajući. Da bi sinteza biodizela pomoću imobilizirane lipaze dala željene rezultate (FAME > 96 %) potrebno je povećati masu imobiliziranog enzima koji se dodaje u reakcijsku smjesu s 735 mg na 1327,39 mg.
Keywordsbiodiesel oil waste oil immobilization lipase Thermoymces lanuginosus
Parallel title (English)Syntesis of biodiesel by immobilized lipase from Thermomyces
Committee MembersMarina Tišma
Sandra Budžaki
Ivica Strelec
Natalija Velić
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeProcess Engineering
Academic title abbreviationmag. ing. proc.
Genremaster's thesis
Language Croatian
Defense date2015-10-30
Parallel abstract (English)
The aim of this study was to immobilize lipase from Thermomyces lanuginosus on the carrier Eupergit CM and perform synthesis by immobilised lipase in batch reactor from fresh edible and waste edible oil. Process of immobilisation was performed during 24, 48, 72 and 96 h in two buffer of different molarity. The best results show immobilisation during 72 h in 0.1 M buffer (the most residual enzyme after immobilisation). The amount of produced biodiesel from fresh edible and waste edible oil was unsatisfactory. Therefore, increase in immobilized enzyme mass from 735 mg to 1327.39 mg is required for achieving desired results (FAME > 96 %) for biodiesel synthesis.
Parallel keywords (Croatian)biodizel ulje otpadno ulje imobilizacija lipaza Thermomyces lanuginosus
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:683729
CommitterIvana Šuvak