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master's thesis
Validacija multimikotoksinske LC-MS/MS metode
2012. urn:nbn:hr:109:706024
Rupčić, Željka
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology

Cite this document

Rupčić, Ž. (2012). Validacija multimikotoksinske LC-MS/MS metode (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:706024

Rupčić, Željka. "Validacija multimikotoksinske LC-MS/MS metode." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2012. https://urn.nsk.hr/urn:nbn:hr:109:706024

Rupčić, Željka. "Validacija multimikotoksinske LC-MS/MS metode." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2012. https://urn.nsk.hr/urn:nbn:hr:109:706024

Rupčić, Ž. (2012). 'Validacija multimikotoksinske LC-MS/MS metode', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:109:706024

Rupčić Ž. Validacija multimikotoksinske LC-MS/MS metode [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2012 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:109:706024

Ž. Rupčić, "Validacija multimikotoksinske LC-MS/MS metode", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:109:706024

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Metadata
TitleValidacija multimikotoksinske LC-MS/MS metode
Title (english)Validation of a multimycotoxin LC-MS/MS method
AuthorŽeljka Rupčić
MentorTomislav Klapec (mentor)
Committee memberDaniela Čačić Kenjerić
Committee memberTomislav Klapec
Committee memberIvica Strelec
Committee memberLjiljana Primorac
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Biochemistry and Toxicology)
Osijek
Defense date and country2012-03-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
Zadatak ovog rada bio je validirati metodu za istovremenu analizu devet mikotoksina korištenjem LC-MS/MS-a. Validacijom ove metode ispitivana je sposobnost metode za određivanje trihotecenskih mikotoksina u uzorcima pšenice. Spektrometar masa je podešen za rad u pozitivnom i negativnom ionizacijskom modu, te je izvršena optimizacija ostalih parametara (napon za lokaliziranje i razbijanje, ulazni napon, kolizijska energija kao i izlazni potencijal kolizijske ćelije). Prije analize obavljena je kalibracija otopinama standarda, te je dokazana prihvatljiva linearnost metode. Uzorak pšenice podvrgnut je ekstrakciji i pročišćavanju na SPE koloni, prije analize na LC-MS/MS-u. Osim linearnosti, ispitivani validacijski parametri su bili repetabilnost, reproducibilnost, limit detekcije (LOD), limit kvantifikacije (LOQ) i iskorištenje. Obzirom na navedene parametre, metoda je zadovoljila zakonsku regulativu, osim vrijednosti iskorištenja za nivalenol, fuzarenon X, T-2 i HT-2 toksin, koje je bilo ispod propisanog minimuma.
Abstract (english)
The aim of this work was to validate a multimycotoxin LC-MS/MS method for simultaneous analysis of nine mycotoxins. The method’s ability to measure trichothechene mycotoxins in wheat was estimated. The mass spectrometer was operated in both positive and negative ionization mode. Other parameters, including declustering potential, focusing potential, entrance potential, collision energy and collision cell exit potential, were optimized as well. Calibration was performed using standard solutions standard and acceptable linearity was obtained. Wheat sample extracts were subjected to SPE column clean-up prior to LC-MS/MS analysis. In addition to linearity, tested validation parameters were also repetability, reproducibility, detection limit (LOD), quantitation limit (LOQ) and recovery. According to the results, this method met regulatory demands, except for nivalenol, fusarenon X, T-2 and HT-2. Recoveries of these toxins were below acceptable ranges.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:706024
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2015-12-14 14:23:04