master's thesis
Influence of Aspergillus flavus mycelium pre-treatment on ultrasonic disintegration efficiency

Tihomir Kovač (2010)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
Metadata
TitleUtjecaj predobrade micelija plijesni Aspergillus flavus na učinkovitost ultrazvučne homogenizacije
AuthorTihomir Kovač
Mentor(s)Ivica Strelec (thesis advisor)
Abstract
Cilj istraživanja bio je odrediti utjecaj predobrade micelija plijesni Aspergillus flavus na učinkovitost ultrazvučne homogenizacije, te optimalne uvjete ultrazvučnog razbijanja kako bi se u potpunosti oslobodio unutarstanični sadržaj, a da pri tome ne dođe do denaturacije proteina i gubitka aktivnosti katalaze. Rezultati praćeni mikroskopskom analizom dezintegriranosti micelija, određivanjem količine proteina u ekstraktu te određivanjem aktivnosti katalaze u ekstraktu su pokazali kako je najučinkovitija predobrada micelija plijesni Aspergillus flavus kombinacija predrazbijanja micelija plijesni kvarcnim pijeskom i enzimske razgradnje stanične stjenke lizozimom pri punom ciklusu i najvišoj amplitudi ultrazvučne homogenizacije u trajanju od 5 minuta. Uz navedenu predobradu i uvjete ultrazvučne homogenizacije u ekstraktu i ispirku taloga je moguće dobiti oko 87% ukupne količine proteina, odnosno oko 88% ukupne količine aktivnosti enzima.
Keywordsmolds Aspergillus flavus pre‐treatment quartz sand lysozyme ultrasonic disintegration
Parallel title (English)Influence of Aspergillus flavus mycelium pre-treatment on ultrasonic disintegration efficiency
Committee MembersLidija Jakobek
Ivica Strelec
Tomislav Klapec
Mirela Planinić
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeProcess Engineering
Academic title abbreviationmag. ing. proc.
Genremaster's thesis
Language Croatian
Defense date2010-07-05
Parallel abstract (English)
Influence of Aspergillus flavus mycelium pre‐treatment on ultrasonic disintegration efficiency, as well as optimisation of ultrasonic disintegration conditions for complete release of intracellular components without protein denaturation and loss of enzyme activity was investigated. Results of ultrasonic disintegration monitored by microscopic analysis, protein quantification and determination of catalase activity has shown that optimal conditions for ultrasonic homogenisation of Aspergillus flavus mycelium are: pre‐treatment combination of quartz sand crashing and lysozyme degradation of cell wall followed by ultrasonic processing during 5 minutes at full cycle and maximal amplitude. Under such conditions of ultrasonic homogenisation extracts obtained after 5 minute homogenization and extract of sediment additionally homogenized for 2 minutes contained 87 % of total proteins, and 88 % of enzyme activity.
Parallel keywords (Croatian)plijesni Aspergillus flavus predobrada kvarcni pijesak lizozim ultrazvučna homogenizacija
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:349419
CommitterIvana Šuvak