master's thesis
Mathematical modelling of Trametes versicolor growth on different carbohydrates

Martina Sudar (2010)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering
Metadata
TitleMatematičko modeliranje procesa rasta Trametes versicolor na različitim šećerima
AuthorMartina Sudar
Mentor(s)Đurđa Vasić-Rački (thesis advisor)
Abstract
Gljiva bijelog truljenja Trametes versicolor proizvodi različite izvanstanične enzime od kojih su najpoznatiji lakaze, lignin peroksidaze i mangan peroksidaze. Lakaze (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) su oksidaze koje kataliziraju oksidaciju supstrata prijenosom elektrona i istovremenom redukcijom kisika u vodu. Ekološki su prihvatljive jer im je za katalitičko djelovanje potreban samo kisik iz zraka, pri čemu kao jedini nusproizvod nastaje voda. Kataliziraju reakcije oksidacije velikog broja supstrata. U ovom radu je istraživan uzgoj gljive bijelog truljenja Trametes versicolor u obliku micelijskih peleta na glukozi, fruktozi i saharozi. Rast biomase u submerznim uvjetima šaržnog uzgoja na glukozi i fruktozi opisan je Monodovim kinetičkim modelom. Prilikom rasta gljive na saharozi, pretpostavljeno je da je hidroliza saharoze do glukoze i fruktoze katalizirana invertazom, što je opisano Michaelis-Menteničinom kinetikom, nakon čega slijedi rast gljive paralelno na glukozi i fruktozi. Nadalje, u radu su provedena istraživanja proizvodnje lakaze na otpadu iz industrije papira. Istraženi su utjecaji sastava hranjive podloge (početne koncentracije micelijskih peleta, koncentracije industrijskog otpada, kvaščevog ekstrakta i peptona) na aktivnost enzima lakaze.
Keywordsmushrooms Trametes versicolor laccase Monod model Michaelis-Menten kinetic model
Parallel title (English)Mathematical modelling of Trametes versicolor growth on different carbohydrates
Committee MembersDamir Magdić
Đurđa Vasić-Rački
Ivica Strelec
Vinko Krstanović
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeProcess Engineering
Academic title abbreviationmag. ing. proc.
Genremaster's thesis
Language Croatian
Defense date2010-07-12
Parallel abstract (English)
White-rot fungus Trametes versicolor produces different extracellular enzymes and the best known are laccase, lignin-peroxidase and manganese-peroxidase Laccases (EC 1.10.3.2, p-diphenol: dioxygen oxidoreductases) are oxidases that catalyze substrate oxidation by electron transfer and simultaneous reduction of oxygen to water. They are environmentally ideal while they require only air for their catalytic function and produce water as the only by product. They have a very wide substrate range. In this work the growth of white-rot fungus Trametes versicolor on glucose, fructose and sucrose in a form of mycelial pellets was investigated. Biomass growth in submerged batch conditions on glucose and fructose was described using the Monod kinetic model. It was assumed that, during the biomass growth on sucrose, sucrose enzymatic decomposition to glucose and fructose is catalyzed with invertase which was described with the Michaelis-Menten kinetics. After the sucrose enzymatic decomposition, Trametes versicolor grows simultaneous on glucose and fructose. Furthermore, in this work the production of laccase using waste from paper industry was investigated. The effect of the nutrient medium composition (initial mycelial pellets concentration, initial industrial waste concentration, yeast and peptone concentration) on laccase activity was studied.
Parallel keywords (Croatian)gljive Trametes versicolor lakaza Monodov model Michaelis-Menteničin kinetički model
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:163939
CommitterIvana Šuvak