prikaz prve stranice dokumenta Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka
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master's thesis
Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka
2010. urn:nbn:hr:109:210313
Eškit, Marina
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology

Cite this document

Eškit, M. (2010). Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:210313

Eškit, Marina. "Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2010. https://urn.nsk.hr/urn:nbn:hr:109:210313

Eškit, Marina. "Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2010. https://urn.nsk.hr/urn:nbn:hr:109:210313

Eškit, M. (2010). 'Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 28 January 2025, https://urn.nsk.hr/urn:nbn:hr:109:210313

Eškit M. Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2010 [cited 2025 January 28] Available at: https://urn.nsk.hr/urn:nbn:hr:109:210313

M. Eškit, "Fluorescencija peptidne frakcije kravljeg i kozjeg mlijeka", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:109:210313

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Metadata
TitleFluorescencija peptidne frakcije kravljeg i kozjeg mlijeka
Title (english)Fluorescence of peptide fraction of cow´s and goat´s milks
AuthorMarina Eškit
MentorIvica Strelec (mentor)
Committee memberJovica Hardi (član povjerenstva)
Committee memberIvica Strelec (član povjerenstva)
Committee memberLidija Jakobek (član povjerenstva)
Committee memberVedran Slačanac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Biochemistry and Toxicology)
Osijek
Defense date and country2010-12-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Food Chemistry
Abstract
Cilj istraživanja bio je ustanoviti može li se fluorescencijom i određivanjem intenziteta fluorescencije peptidne frakcije mlijeka, te u njoj prisutne količine ukupnog amino dušika kao i peptida/proteina detektirati zamjena dijela kozjeg sa kravljim mlijekom, te odrediti donji limit detekcije. Analizirane su peptidne frakcije kravljeg mlijeka (sirovo, pasterizirano, UHT i rekonstiturano) i kozjeg mlijeka (sirovo, UHT). Peptidne frakcije dobivene su taloženjem sa 40 % T CA te centrifugiranjem pri 3000 g. Analizom spektra fluorescencije i određivanjem intenziteta fluorescencije dokazano je da se peptidne frakcije kravljeg i kozjeg mlijeka razlikuju. Spektri fluorescencije peptidne frakcije kravljeg i kozjeg mlijeka pokazuju dva vrha fluorescencije. Dominantni vrh fluorescencije peptidne frakcije kravljeg mlijeka nalazi se pri 280/425 nm, a kozjeg mlijeka 290/390 nm, što je naknadno dokazano normalizacijom intenziteta fluorescencije. Peptidna frakcija kozjeg mlijeka sadrži više ukupnog amino dušika i pokazuje pet do deset puta veći intenzitet fluorescencije pri 290/340 i 290/390 nm za razliku od kravljeg mlijeka. Donji limit detekcije za određivanje patvorenja kozjeg mlijeka sa kravljim pri 290/340 nm i pri 290/390 nm iznosio je 10 %.
Abstract (english)
The aim of the study was to determine the possibility of detection of goat milk alduteration with cow’s milk by fluorescence measurement of non-protein nitrogen (NPN) fraction of milk, and in the content of total amino nitrogen, as well as to determine the lower limit of substitution detection. NPN fractions were obtained by precipitation with 40% TCA followed by the centrifugation at 3000 g. The NPN fractions of cow's milk (raw, pasteurized, UHT and reconstitution) and goat's milk (raw, UHT) were investigated. The analysis of the fluorescence spectrum and intensity of fluorescence showed that cow and goat milk NPN fractions differs in fluorescence intensity and fluorescence peaks wavelenghts. Fluorescence spectra of peptide fractions of cow's and goat's milk showed two peaks of fluorescence. Dominant peak of fluorescence of peptide fractions of cow's milk was centered at 280/425 nm and at 290/390 nm for goat milk. Peaks were confirmed by normalization of fluorescence intensities. NPN fraction of goat's milk contains higher amount of total amino nitrogen compared to cow’s milk. Fluorescence intenisity of goat’s milk NPN fractions were five to ten-fold higher compared to intensities of cow’s milk NPN fractions. The lower limit of detection for the determination of adulteration of goat milk with cow's milk at 290/340 and at 290/390 nm was 10 %.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:210313
ProjectNumber: 113-1130473-0334
Title: Sinergističke smjese u antifungalnoj i antimikotoksigenoj zaštiti hrane
Title: Synergistic mixtures in antifungal and antimycotoxigenic food protection
Leader: Tomislav Klapec
Jurisdiction: Croatia
Funder: MZOS
Funding stream: ZP
ProjectNumber: 113-1130475-2722
Title: Unapređenje kakvoće, trajnosti i funkcionalnosti proizvoda na bazi pšenice
Title: Improvement of quality, shelf life and functionality of wheat-based products
Leader: Daliborka Koceva Komlenić
Jurisdiction: Croatia
Funder: MZOS
Funding stream: ZP
Study programmeTitle: Food Science and Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani (magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2015-12-15 14:44:19