master's thesis
Design of a bioreactor for microorganism cultivation in semi-solid and solid substrates

Ivana Ćurković (2014)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Process Design and Construction Materials
Metadata
TitleProjektiranje bioreaktora za uzgoj mikroorganizama na polučvrstim i čvrstim supstratima
AuthorIvana Ćurković
Mentor(s)Darko Velić (thesis advisor)
Marina Tišma (thesis advisor)
Abstract
Fermentacije na čvrstim nosačima (eng. Solid State Fermentations, SSF) su procesi koji podrazumijevaju rast mikroorganizama na vlažnim čvrstim nosačima bez prisustva slobodne vode, pri čemu čvrsti nosači mogu ujedno biti i supstrati za rast, tzv. neinertni nosači. Jedan od primjera neinertnih nosača u SSF su različiti lignocelulozni materijali, pri čemu se SSF primjenjuje za mikrobiološku razgradnju ovih materijala. Lignocelulozni materijali predstavljaju važan obnovljivi izvor energije i kao otpad u velikim količinama nastaju u poljoprivrednoj, prehrambenoj i drvnoj industriji. Nadalje, ovi materijali zanimljivi su i kao potencijalna sirovina u biotehnološkoj proizvodnji, pri čemu najveći problem predstavlja teška razgradnja lignoceluloznih sastavnica (celuloze, hemiceluloze i lignina) do jednostavnih šećera. Fermentacije na čvrstim nosačima uspješno se primjenjuju za mikrobiološku razgradnju lignocelulozne biomase, pri čemu se kao radni mikroorganizam najčešeće koriste gljive bijelog truljenja koje imaju sposobnost razgradnje lignoceluloznih sastavnica do fermentabilnih šećera. U ovom radu, gljiva bijelog truljenja, Trametes versicolor uzgajana je na repinim rezancima kao čvrstom supstratu i nosaču u reaktorskom sustavu pri ambijentalnoj temperaturi tijekom 30 dana. Istraživan je utjecaj T. versicolor na razgradnju repinih rezanaca. Tijekom fermentacije praćene su promjene procesnih parametra (temperatura i relativna vlažnost u sustavu), kao i gubitak na masi lignoceluloznog supstrata te promjena boje i kemijskog sastava supstrata. Povećanje temperature i relativne vlažnosti u sustavu, kao i gubitak na masi supstrata od 21,33 % ukazuju na intenzivan rast i aktivnost radnog mikroorganizma. Nadalje, konverzija celuloze od 64,65 % i pentozana od 42,39 % nakon 30 dana uzgoja također ukazuju na aktivnost radnog mikroorganizma i produkciju enzima odgovornih za razgradnju lignoceluloznih sastavnica. Na osnovi provedenog eksperimenta i dobivenih rezultata projektiran je horizontalni bioreaktor s kombiniranim sustavom miješanja.
Keywordsbioreactor design horizontal bioreactor lignocellulosic materials solid state fermentation Trametes versicolor
Parallel title (English)Design of a bioreactor for microorganism cultivation in semi-solid and solid substrates
Committee MembersNatalija Velić
Darko Velić
Marina Tišma
Mirela Planinić
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Process Engineering
Sub-department of Process Design and Construction Materials
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeProcess Engineering
Academic title abbreviationmag. ing. proc.
Genremaster's thesis
Language Croatian
Defense date2014-04-08
Parallel abstract (English)
Solid State Fermentation (SSF) is a process where the microorganisms are grown on moist solid substrates in the absence of free water, where the solid carrier can also be a substrate for the microorganism growth, so-called, non-reactive substrate. An example of non-reactive substrates in SSF are different lignocellulosic materials, where in the SSF is applied for the microbiological decomposition of these materials. Lignocellulosic materials represent an important renewable energy source occurring as waste in large quantities in the agricultural, food and timber industries. These materials, however,could have application in biotechnological production, but the biggest problem is the severe degradation of lignocellulosic components (cellulose, hemicellulose and lignin) to simple sugars. SSF is successfully applied for microbial degradation of the lignocellulose, where in white rot fungi are the most often used microorganisms. In this study white rot fungi, Trametes versicolor was cultivated on sugar beet pulp as a solid substrate and the carrier in the reactor at ambient temperature for 30 days. The possibility of T. versicolor to degrade beet pulp was investigated. During fermentation, the change of process parameters was observed (temperature and relative humidity in the system), as well as the loss of substrate mass,change of the color and chemical composition. Temperature and relative humidity in crease as well as the loss of substrate mass (21,33%) indicate strong growth and the activity of working microorganisms. Furthermore, the conversion of cellulose 64.65% and 42.39% of pentosans after 30 days of cultivation also indicates the activity of working microorganism and production of enzymes responsible for degradation of lignocellulosic components. A horizontal bioreactor with combined interference system was designed based on obtained results.
Parallel keywords (Croatian)fermentacija na čvrstim nosačima horizontalno-cilindrični stakleni reaktor lignocelulozni materijal projektiranje bioreaktora Trametes versicolor
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:911356
CommitterSanda Hasenay