master's thesis
Deoxynivalenol metabolism and analysis of its biomarkers

Dijana Podravac (2016)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Food and Nutrition Research
Sub-department of Nutrition
Metadata
TitleMetabolizam deoksinivalenola i analiza njegovih biomarkera
AuthorDijana Podravac
Mentor(s)Bojan Šarkanj (thesis advisor)
Abstract
Mikotoksini su sekundarni metaboliti plijesni koje nastaju na polju ili tijekom skladištenja uroda te često kontaminiraju hranu. Deoksinivalenol (DON) i njegovi maskirani oblici su mikotoksini koje proizvode plijesni vrste Fusarium i najčešće kontaminiraju kukuruz, pšenicu, ječam i zob. Zbog svojeg štetnog utjecaja na zdravlje, potrebno je raditi na razvoju novih metoda njegovog određivanja kako bi se olakšala procjena rizika i procjena izloženosti. U ovom radu je istražena stopa izlučivanja i glukuronidacije DON-a pomoću biomarkera iz urina 49 dobrovoljaca nakon konzumacije namirnica s poznatim koncentracijama DON-a i njegovih metabolita. Za analizu urina korištena je multimikotoksinska metoda ultra visokodjelotvorne tekućinske kromatografije – tandemske masene spektrometrije (UHPLC-MS/MS). Analiza prvog jutarnjeg urina nakon dva dana pripreme za intervencijsku prehranu provedena je da bi se potvrdilo nepostojanje DON-a u urinu ispitanika koji bi mogao utjecati na rezultate prehrane jer se DON eliminira iz organizma unutar 24 sata. 24-satni urin trećeg intervencijskog dana potvrdio je da se većina DON-a izluči u obliku DON-15-glukuronida, nešto manje u slobodnom obliku, a ostatak u obliku DON-3-glukuronida koji kod nekih ispitanika nije bio ni detektiran zbog preniskih koncentracija DON-a u unesenoj hrani. Stopa izlučivanja bila je 82%, a stopa konjugacije 89 %.
Keywordsmycotoxins deoxynivalenol biomarkers LC-MS/MS glucuronides urine
Parallel title (English)Deoxynivalenol metabolism and analysis of its biomarkers
Committee MembersTomislav Klapec (committee chairperson)
Bojan Šarkanj (committee member)
Daniela Čačić Kenjerić (committee member)
Ivana Flanjak (committee member)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Food and Nutrition Research
Sub-department of Nutrition
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Food Microbiology
Study programme typeuniversity
Study levelgraduate
Study programmeFood Science and Nutrition
Academic title abbreviationmag. nutr.
Genremaster's thesis
Language Croatian
Defense date2016-07-15
Parallel abstract (English)
Mycotoxins are secondary metabolites of molds that occur in the field or during storage of crops and often contaminate food. Deoxynivalenol (DON) and its masked forms are mycotoxins produced by Fusarium molds and commonly contaminate corn, wheat, barley and oats. Due to its harmful effects on health, it is necessary to work on the development of new methods of determining how to facilitate exposure and risk assessment. This work investigated the rate of excretion and glucuronidation of DON using biomarkers from urine of 49 volunteers after they consumed foods with known concentrations of DON and its metabolites. Multi-mycotoxin ultra high-performance liquid chromatography - tandem mass spectrometry (UHPLC-MS / MS) method was used for urine analysis. Analysis of the first morning urine after two days of preparation for nutritional intervention was carried out to confirm absence of DON in the urine of subjects that could affect the results of the intervention because DON is eliminated from the body within 24 hours. 24-hour urine from the third intervention day confirmed that the majority of DON was excreted in the form of DON-15-glucuronide, a little less in the free form, and the rest in the form of DON-3-glucuronide which was not detected in some subjects because of low DON concentrations in their food. The rate of excretion and conjugation were 82% and 89%, respectively.
Parallel keywords (Croatian)mikotoksini deoksinivalenol biomarkeri LC-MS/MS glukuronidi urin
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:301649
CommitterSanda Hasenay