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master's thesis
Kinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja
2015. urn:nbn:hr:109:537050
Ćurić, Ana
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering

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Ćurić, A. (2015). Kinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:537050

Ćurić, Ana. "Kinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2015. https://urn.nsk.hr/urn:nbn:hr:109:537050

Ćurić, Ana. "Kinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2015. https://urn.nsk.hr/urn:nbn:hr:109:537050

Ćurić, A. (2015). 'Kinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 21 January 2025, https://urn.nsk.hr/urn:nbn:hr:109:537050

Ćurić A. Kinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2015 [cited 2025 January 21] Available at: https://urn.nsk.hr/urn:nbn:hr:109:537050

A. Ćurić, "Kinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:109:537050

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Metadata
TitleKinetička karakterizacija lipaze iz Thermomyces lanuginosus i njezina upotreba u proizvodnji biodizela iz suncokretovog ulja
Title (english)Kinetic characterization of lipase from thermomyces lanuginosus and its use in the production of biodiesel from sunflower oil
AuthorAna Ćurić
MentorMarina Tišma (mentor)
Committee memberIvica Strelec
Committee memberMarina Tišma
Committee memberSandra Budžaki
Committee memberMirela Planinić
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Thermodynamics and Reaction Engineering)
Osijek
Defense date and country2015-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
Cilj ovoga rada bio je razviti kinetički model hidrolize p-NPP-a katalizirane lipazom koristeći tri različita otapala (acetonitril, metanol i etanol), procijeniti kinetičke parametre procesa (Vm i Km), istražiti utjecaj koncentracije enzima, vrste otapala i temperature na aktivnost lipaze te provesti jednostupanjsku i četverostupanjsku reakciju enzimske sinteze biodizela iz jestivog suncokretovog ulja. Mjerenjima početne reakcijske brzine pokazano je da su najvede volumne aktivnosti lipaze postignute koristeći 5 % (g/g) koncentraciju enzima u pokusima sa svim ispitanim otapalima (Vm,etanol = 1373,852 U/mL, Vm,acetonitril = 318,817 U/mL, Vm,metanol = 1141,068 U/mL). Najveći afinitet prema supstratu (p-NPP) lipaza pokazuje u sustavu s acetonitrilom kao otapalom (Km = 1,0217 mM). U jednostupanjskim i četverostupanjskim procesima proizvodnje biodizela iz jestivog suncokretovog ulja pri različitim temperaturama (T = 40 – 60 °C), najviši udio metil estera postignut je pri T = 40 °C. U jednostupanjskoj reakciji udio metil estera iznosi 94,9 % nakon 96 sati, dok je za isto vrijeme u četverostupanjskoj reakciji udio metil estera iznosio 89,0 %.
Abstract (english)
The aim of this study was to develop a kinetic model for hydrolysis of p-NPP catalyzed with lipase using three different solvents (acetonitrile, methanol and ethanol), to evaluate the kinetic parameters (Vm and Km), to investigate the influence of enzyme concentration, solvent type and temperature on lipase activity and to perform single step and four step reaction of biodiesel synthesis from edible sunflower oil catalyze by lipase. The initial reaction rate measurements shown that the highest volume activity of lipase was achieved using 5% (g/g) concentration of enzyme in the assays with all tested solvents (Vm, ethanol = 1373.852 U/mL, Vm, acetonitrile = 318.817 U/mL, Vm,methanol = 1141.068 U/mL). The highest affinity to the substrate (p-NPP) lipase shows in reaction with acetonitrile (Km = 1.0217 mM). In the single step and four step process of biodiesel production from edible sunflower oil at different temperatures (T = 40 – 60 °C), the highest fatty acids methyl esters share were achieved at T = 40 °C. 94.9 % fatty acids methyl esters share was achieved in single step reaction after 96 h and 88.9 % in four step reaction for the same duration.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:537050
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2015-09-28 15:53:56