master's thesis
Determination of sugar concentration during biological treatment of grape pomace with Trametes versicolor

Marko Jovanovac (2017)
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Unit Operations
Metadata
TitleOdređivanje koncentracije šećera tijekom biološke obrade tropa grožđa pomoću Trametes versicolor
AuthorMarko Jovanovac
Mentor(s)Ana Bucić-Kojić (thesis advisor)
Abstract
Trop grožđa je otpad koji zaostaje nakon proizvodnje vina, te se u većini slučajeva bez dodatne obrade odlaže na deponije, u vinograde kao dodatak gnojivu ili se u krajnjem slučaju spaljuje. Primjenom europskih regulativa, takav način zbrinjavanja je neprihvatljiv. Trop grožđa je energetski i nutritivno visokovrijedna sirovina budući da predstavlja bogat izvor biološki aktivnih fenolnih spojeva te šećera koji su između ostalog neophodni u proizvodnji visokovrijednih produkata, primjerice bioetanola i bioplina. U ovom radu provedena je kruto-tekuća ekstrakcija šećera (uvjeti: temperatura 30 °C, vrijeme 30 min, broj okretaja tresilice 170 rpm, omjer kruto-tekuće 25 mL/g) iz neobrađenog i biološki obrađenog tropa grožđa gljivom bijelog truljenja Trametes versicolor u uvjetima fermentacije na čvrstim nosačima s ciljem praćenja koncentracije i potrošnje šećera tijekom 15 dana fermentacije. Najzastupljeniji šećer u analiziranim ekstraktima biološki neobrađenog tropa grožđa bila je fruktoza (7,09 mg/g), a potom su slijedile glukoza (5,72 mg/g), maltotrioza (3,88 mg/g.), maltoza (2,48 mg/g) i saharoza (0,63 mg/g). Praćenjem koncentracije pojedinačnih šećera u ekstraktima biološki obrađenog tropa grožđa tijekom 15 dana utvrđeno je da se najznačajnije promjene u koncentraciji promatranih šećera događaju u prvih 6-7 dana fermentacije gdje su se udjeli fruktoze, glukoze, maltotrioze i saharoze smanjivali u odnosu na početnu vrijednost, a udio maltoze je bio konstantan prvih pet dana fermentacije te se potom povećavao nakon šestog dana fermentacije.
Keywordsgrape pomace Trametes versicolor
Parallel title (English)Determination of sugar concentration during biological treatment of grape pomace with Trametes versicolor
Committee MembersMarina Tišma (committee chairperson)
Ana Bucić-Kojić (committee member)
Mirela Planinić (committee member)
Sandra Budžaki (committee member)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Lower level organizational unitsDepartment of Process Engineering
Sub-department of Unit Operations
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Food Technology
Engineering
Study programme typeuniversity
Study levelgraduate
Study programmeProcess Engineering
Academic title abbreviationmag. ing. proc.
Genremaster's thesis
Language Croatian
Defense date2017-04-11
Parallel abstract (English)
Grape pomace is a waste remaining after wine production, and in most cases it ends up in landfills without previous treatment, in vineyards as a fertilizers or ultimately, incinerated. By European regulations, this method of disposal is unacceptable. Grape pomace is energy and nutritionally valuable raw material as it represents a rich source of biologically active phenolic compounds and sugars, which is necessary in the production of high value products, such as bioethanol and biogas. In this paper, solid-liquid extraction(conditions: temperature 30 °C, 30 minutes, speed shaker 170 rpm, the liquid-solid ratio 25 mL/g) of sugars from untreated and biologically treated grape pomace with Trametes versicolor was performed. The most common sugar in extract of untreated grape pomace was fructose (7.09 mg/g) followed by glucose (5.72 mg/g), maltotriose (3.88 mg/g), maltose (2.48 mg/g) and sucrose (0.63 mg/g).Concentration monitoring of the individual sugars in biologically treated grape pomace extracts during 15 days of fermentation showed that the most significant changes occurred in first 6 – 7 days of fermentation. Fructose, glucose, maltotriose and sucrose content was decreased in relation to initial value and maltose content was constant up to 5th day of fermentation and after 6th day of fermentation it was increased.
Parallel keywords (Croatian)trop grožđa Trametes versicolor
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:109:988927
CommitterIvana Šuvak