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master's thesis
Utjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva
2015. urn:nbn:hr:109:209442
Kajić, Dragana
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Unit Operations

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Kajić, D. (2015). Utjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:209442

Kajić, Dragana. "Utjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2015. https://urn.nsk.hr/urn:nbn:hr:109:209442

Kajić, Dragana. "Utjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2015. https://urn.nsk.hr/urn:nbn:hr:109:209442

Kajić, D. (2015). 'Utjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 21 February 2025, https://urn.nsk.hr/urn:nbn:hr:109:209442

Kajić D. Utjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2015 [cited 2025 February 21] Available at: https://urn.nsk.hr/urn:nbn:hr:109:209442

D. Kajić, "Utjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:109:209442

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TitleUtjecaj biološke obrade kukuruzne silaže pomoću Trametes versicolor na ekstrakciju fenolnih spojeva
Title (english)Influence of biological pretreatment of corn silage with Trametes versicolor on phenolic compounds extraction
AuthorDragana Kajić
MentorAna Bucić-Kojić (mentor)
Committee memberMirela Planinić
Committee memberAna Bucić-Kojić
Committee memberMarina Tišma
Committee memberSandra Budžaki
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Unit Operations)
Osijek
Defense date and country2015-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
U ovome radu ispitan je utjecaj procesnih uvjeta (broj plagova - P, masa kukuruzne silaže - m, pH i vrijeme fermentacije) biološke obrade kukuruzne silaže na ekstrakciju fenolnih spojeva (ukupnih fenolnih spojeva, ukupnih flavonoida i fenolnih kiselina: p-kumarinske, ferulične i kafeinske) metodom odzivnih površina prema Box-Behnkenovom planu pokusa. Biološka obrada uzoraka provedena je u uvjetima fermentacije na čvrstim nosačima, pomodu gljive bijelog truljenja Trametes versicolor. Biološki obrađeni uzorci osušeni su pri 45 °C tijekom 48 h, te su nakon toga podvrgnuti ekstrakciji (uvjeti ekstrakcije: 50% otopina etanola/80 °C/120 min/200 rpm). U ekstraktima biološki obrađene kukuruzne silaže spektrofotometrijskim metodama analizirani su ukupni fenolni spojevi (Folin-Ciocalteuovom metodm - 9,47 mgGAE/gs.t. i Prussian-Blue metodom – 0,55 mgGAE/gs.t.) i ukupni flavonoidi (1,47 mgCE/gs.t.) te su HPLC metodom određene individualne fenolne kiseline (p-kumarinska: 0,36 mg/gs.t, ferulična: 0,27 mg/gs.t i kafeinska: 0,14 mg/gs.t.). Broj plagova gljive T. versicolor i masa kukuruzne silaže tijekom procesa biološke obrade imali su najvedi utjecaj na prinos fenolnih tvari. Za opisivanje procesa biološke obrade kukuruzne silaže korišten je kvadratni polinom koji je dobro opisivao ispitivani proces s ciljem dobivanja maksimalnog prinosa analiziranih fenolnih tvari. Ipak, najbolja slaganja modelom predviđenih podataka i eksperimentalnih rezultata uočena su kod kafeinske kiseline. Metodom odzivnih površina određeni su optimalni uvjeti biološke obrade kukuruzne silaže s ciljem dobivanja maksimalnog prinosa ispitivanih spojeva. Kod kafeinske kiseline optimalni uvjeti su: 9 plagova, 40 g kukuruzne silaže, pH 9 i 10 dana fermentacije. Pri tim uvjetima predviđeni maksimalni prinos kafeinske kiseline koji bi se mogao ekstrahirati je 0,14 mg/gs.t. što je u skladu s eksperimentalno određenim maksimalnim prinosom kafeinske kiseline.
Abstract (english)
The influence of process conditions (number of plugs - P, mass of corn silage – m, pH and duration of fermentation) in biological treatment of corn silage on extractability of phenolic compounds (total phenols, total flavonoids and phenolic acids: p-coumaric acid, fenolic acid and caffeic acid) was investigated using response surface methodology (Box-Behneken design). Biological treatment was performedin condition of solid-state fermentation with white-rot fungi Trametes versicolor. Extraction of phenolic compounds from dried (at 45 °C/ 48h by oven) biollogically treted samples was performed using 50% aqueous ethanol solution, 80°C during 120 min. Total phenolic compounds (FC method – 9.47 mgGAE/gdb and PB method – 0.55 mgGAE/gdb) and total flavonoids (1.47 mgCE/gdb) from biological treated samples were determined by spectrophotometric methods, while individual phenolic acids (p-coumaric: 0.36 mg/gdb, ferulicacid: 0.27 mg/gdb i caffeic acid: 0.14 mg/gdb) were analyzed by HPLC device. Number of Trametes versicolor plugs and mass of corn silage had the highest impact on extraction yield of phenolic compounds. Quadratic polinom was used to describe biological treatment of corn silage in order to maximize phenolic compounds yield. However, the best matching of predicted data and experimental results was noticed with caffeic acid. Response surface methodology was used for determination of optimal conditions for biological treatment of corn silage in order to maximize the analyzed compounds yield. Optimal conditions for maximum caffeic acid yield were: 9 plugs, 40g of corn silage, pH 9 and 10 days of fermentation. However, in these conditions maximum caffeic acid yield that could be extracted is 0.14 mg/gdb, which is in accordance with maximum experimental caffeic acid yield.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:209442
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2015-09-28 10:33:26